FastQCFastQC Report
Sun 20 Nov 2022
CO_010-COL.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_010-COL.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12219
Sequences flagged as poor quality0
Sequence length25-353
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC5124.190195597021033No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG4353.5600294623127917No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC4233.461821753007611No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG3883.175382600867501No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG3863.1590146493166382No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG3673.0035191095834355No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG3332.725263933218758No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA3322.717079957443326No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG2211.8086586463704066No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG2191.7922906948195434No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA2171.77592274326868No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2101.718634912840658No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1841.5058515426794337No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1771.4485637122514117No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1741.4240117849251166No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC1721.4076438333742531No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC1681.3749079302725264No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT1631.333988051395368No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1591.301252148293641No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1501.2275963663147558No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG1451.186676487437597No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1421.1621245601113022No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT1401.1457566085604387No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT1391.1375726327850069No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA1381.1293886570095752No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1361.113020705458712No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1351.1048367296832802No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT1301.0639168508061216No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1271.0393649234798266No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1261.0311809477043947No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC1251.022996971928963No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA1251.022996971928963No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG1220.998445044602668No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC1200.9820770930518046No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA1190.9738931172763728No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT1160.9493411899500778No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG1040.8511334806448972No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1000.8183975775431704No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT980.8020296259923071No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT950.777477698666012No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT950.777477698666012No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT900.7365578197888534No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT890.7283738440134216No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC810.6629020378099681No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT810.6629020378099681No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC800.6547180620345364No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG790.6465340862591046No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT780.638350110483673No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT710.5810622800556511No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG660.5401424011784924No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT640.5237744496276291No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC620.5074064980767656No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC610.499222522301334No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT600.4910385465259023No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG600.4910385465259023No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG590.4828545707504706No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT590.4828545707504706No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG580.4746705949750389No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG570.4664866191996072No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT560.4583026434241755No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT540.441934691873312No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT530.4337507160978803No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA520.4255667403224486No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA500.4091987887715852No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC490.40101481299615355No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA490.40101481299615355No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG480.3928308372207218No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT410.3355430067926999No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG400.3273590310172682No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG370.3028071036909731No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG350.2864391521401097No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG330.2700712005892462No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG320.26188722481381455No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC290.23733529748751944No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT280.22915132171208774No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT200.1636795155086341No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA190.15549553973320238No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC160.13094361240690727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)